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ORIENTAL INSTRUMENTS CO., LTD.
Sagamihara, Japan
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Electophoresis

Precast Gel (Long Life Gel)



Precast Gel (Long Life Gel)
for Electrophoresis - Choju Gel


  • Remarkably long life - 12 months in cool and dark storage condition.
  • Reasonable price, high resolution and high reproducibility.
  • Sharp electrophoresis pattern is obtainable by adoption of glass plate.
  • Plate size: 100 x 100mm.
  • Electrophoresis from DNA(nucleic acid) to Protein are achieved.



Selection of Gel


In case of the sample to be analyzed is in a certain fixed range, or molecular weight of the sample is in similar range, use Uniform Gel. When the sample to be analyzed is of molecular weight in wide range, Gradient Gel is recommendable.






Features

  • Without using neurotoxic acrylamide monomer, electrophoresis analysis can be performed by using this Gel.
  • The life of Gel can be guaranteed for 12 months(365 days) in cold storage(+4ºC) condition.
  • The size is for 100×100mm glass plate, and it can be used for most of electrophoresis bath.
  • By adding SDS with electrophoresis buffer, it is used for SDS PAGE.



Operation of Electrophoresis Analysis

  1. Pour electrophoresis buffer till +(plus) Electrode is immersed.
    Protein: Use the buffer with SDS.
    Nuclein acdi(DNA): Use the buffer without SDS.
  2. Set up Gel plate in electrophoresis bath, then revove air bubbles remained at the bottom of Gel by leaning the electrophresis bath.
  3. Fill buffer till the top of Gel plate.
  4. Pour sample for analysis into well.
  5. Electrophresis transmittance condition: 20mA/plate Approx. 70 min.
  6. Dyeing liquid: CBB dyeing or Ag dyeing liquid.



Preparation of Electrophoresis Buffer

25mMol Tris, 192mMol Glycine, 0.1%
SDS Tris 9.08g
Glycine 43.23g
SDS 3.00g Fill up to totally 3 Litre with distilled water.




UNIFORM GEL for Protein, Popular type (10 ea./box)
Plate size : 100 x 100mm

Item No. Gel Concen-
tration (%)
Range of molecular
weight cut off
No. of wells W or W/O Comb Period of
storage (at 4ºC)
HON-075-13 7.5 50,000-200,000 13 With 12 months
HON-075-17 17
HON-100-13 10 20,000-150,000 13
HON-100-17 17
HON-125-13 12.5 20,000-100,000 13
HON-125-17 17
HON-125-2D 2D Without

† Minimum order: 5 boxes



UNIFORM GEL for Protein, Special order type (10 ea./box)
Plate size: 100 x 100mm

Item No. Gel Concen-
tration (%)
Range of molecular
weight cut off
No. of wells W or W/O Comb Period of
storage (at 4ºC)
HON-150-13 15 6,000-100,000 13 With 12 months
HON-150-17 17

† Minimum order: 5 boxes



GRADIENT GEL for Protein, Popular type (10 ea./box)
Plate size : 100 x 100mm

Item No. Gel Concen-
tration (%)
Range of molecular
weight cut off
No. of wells W or W/O Comb Period of
storage (at 4ºC)
HOG-0520-13 5-20 15,000-300,000 13 With 12 months
HOG-0520-17 17
HOG-0520-2D 2D Without
HOG-1020-13 10-20 10,000-150,000 13 With
HOG-1020-17 17

† Minimum order: 5 boxes




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